Biochemical characterization of Alanine racemase- a spore protein produced by Bacillus anthracis
نویسندگان
چکیده
منابع مشابه
Characterization of a major Bacillus anthracis spore coat protein and its role in spore inactivation.
A major Bacillus anthracis spore coat protein of 13.4 kDa, designated Cot alpha, was found only in the Bacillus cereus group. A stable ca. 30-kDa dimer of this protein was also present in spore coat extracts. Cot alpha, which is encoded by a monocistronic gene, was first detected late in sporulation, consistent with a sigma(K)-regulated gene. On the basis of immunogold labeling, the protein is ...
متن کاملProcessing, assembly and localization of a Bacillus anthracis spore protein.
All Bacillus spores are encased in macromolecular shells. One of these is a proteinacious shell called the coat that, in Bacillus subtilis, provides critical protective functions. The Bacillus anthracis spore is the infectious particle for the disease anthrax. Therefore, the coat is of particular interest because it may provide essential protective functions required for the appearance of anthr...
متن کاملGlutamate Racemase Mutants of Bacillus anthracis.
UNLABELLED D-Glutamate is an essential component of bacterial peptidoglycan and a building block of the poly-γ-D-glutamic acid (PDGA) capsule of Bacillus anthracis, the causative agent of anthrax. Earlier work suggested that two glutamate racemases, encoded by racE1 and racE2, are each essential for growth of B. anthracis, supplying D-glutamic acid for the synthesis of peptidoglycan and PDGA ca...
متن کاملCharacterization of a Bacillus anthracis spore coat-surface protein that influences coat-surface morphology.
Bacterial spores are encased in a multilayered proteinaceous shell, called the coat. In many Bacillus spp., the coat protects against environmental assault and facilitates germination. In Bacillus anthracis, the spore is the etiological agent of anthrax, and the functions of the coat likely contribute to virulence. Here, we characterize a B. anthracis spore protein, called Cotbeta, which is enc...
متن کاملStudies on spore germination: its independence from alanine racemase activity.
The demonstration in the past few years that simple nutrients are sufficient to enable a rapid germination of spores from aerobic bacteria has stimulated an interest in the biochemical reactions involved. Glucose, adenosine, and certain amino acids, added singly or in combination, were found by a number of workers to be sufficient to lead to over 90 per cent germination of spores from a number ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: BMB Reports
سال: 2009
ISSN: 1976-6696
DOI: 10.5483/bmbrep.2009.42.1.047